Comparative analysis of genome-wide transcriptional responses to continuous heat stress in Pleurotus tuoliensis
- Author: mycolabadmin
- 11/2/2023
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Summary
Researchers studied how oyster mushrooms respond to heat stress at the genetic level by analyzing which genes turn on and off when exposed to different temperatures. They found that at moderate heat (32°C), the mushrooms could maintain normal growth, but at severe heat (36°C), growth almost completely stopped. The study identified specific genes related to heat shock proteins and cell membrane composition that appear crucial for helping mushrooms survive heat, which could eventually help farmers grow heat-resistant mushroom varieties.
Background
Temperature is critical for mushroom cultivation, and heat stress significantly impairs mycelial growth and fruiting body development. Pleurotus tuoliensis is a novel oyster mushroom with high pharmaceutical value but is highly sensitive to heat stress, with temperatures above 35°C causing growth inhibition and increased pathogen susceptibility.
Objective
To analyze genome-wide transcriptional responses in Pleurotus tuoliensis mycelium under continuous heat stress at 32°C (moderate) and 36°C (severe) compared to control conditions, and identify critical genes and pathways involved in thermotolerance acquisition.
Results
2,724 differential expressed genes were identified across three pairwise comparisons, with 82% showing consistent regulation trends under both heat stress conditions. Nine overlapping GO terms and one overlapping KEGG pathway were identified across all comparisons, including cell communication, amino acid metabolism, and ergosterol biosynthesis. HSP10 and HSP60 genes were significantly upregulated, and 11 ergosterol biosynthesis genes showed altered expression patterns under heat stress.
Conclusion
Heat stress triggers robust transcriptional responses in P. tuoliensis involving multiple biological processes and pathways. Genes related to cell cycle regulation, ergosterol biosynthesis, and heat shock proteins appear critical for thermotolerance acquisition. Further investigation of ergosterol biosynthesis and its connection to membrane composition may provide insights for developing thermally tolerant mushroom strains.
- Published in:AMB Express,
- Study Type:Experimental Research,
- Source: PMID: 37917251, DOI: 10.1186/s13568-023-01630-y