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Chromosome-Level Genome Assembly of Trichoderma cornu-damae Using Hi-C Data

Summary

Researchers created a complete genetic blueprint of a poisonous mushroom called Trichoderma cornu-damae that can be mistaken for medicinal mushrooms. Using advanced DNA sequencing techniques, they mapped all seven chromosomes and identified over 8,500 genes in this toxic fungus. This genetic map will help scientists understand how the mushroom produces dangerous toxins that harm rapidly growing cells. The work provides important information for identifying and studying this poisonous species.

Background

Trichoderma cornu-damae is a poisonous mushroom found in Korea, Japan, China, and Indonesia that produces trichothecene mycotoxins. The mushroom is easily confused with edible or medicinal species like Ganoderma lucidum or Cordyceps militaris due to its immature appearance.

Objective

To construct a high-quality chromosome-level genome assembly of T. cornu-damae using Hi-C sequencing data to better understand the genomic structure and organization of this toxic fungus.

Results

A chromosome-level genome assembly consisting of 7 scaffolds spanning 37.3 Mbp was generated with a scaffold N50 value of 6.5 Mbp and 96.6% of input assembly retained. Gene prediction resulted in 8,544 genes with 97.6% complete BUSCOs, indicating high-quality genome annotation.

Conclusion

The chromosome-level genome assembly of T. cornu-damae provides a valuable genomic resource for understanding the biology of this toxic mushroom. The high-quality assembly and comprehensive gene annotation will facilitate further research on trichothecene biosynthesis and toxin production mechanisms.
  • Published in:Microbiology Resource Announcements,
  • Study Type:Genome Assembly and Annotation,
  • Source: 10.1128/mra.00142-22; PMID: 36197288
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