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Characterization and Expression Pattern Analysis of Pheromone Receptor-Like Genes in Winter Mushroom Flammulina filiformis

Summary

This research examined special genes called pheromone receptors in winter mushrooms that help control how the mushrooms grow and develop. These genes act like cellular switches that respond to various environmental signals and help coordinate mushroom development. Understanding how these genes work could help improve mushroom cultivation. Impacts on everyday life: • Could lead to better methods for growing edible mushrooms • May help improve mushroom crop yields for farmers • Could enable development of more resilient mushroom varieties • May contribute to more efficient commercial mushroom production • Could help reduce costs of mushroom cultivation

Background

Pheromone receptor-like genes (PRLGs) are part of the G protein-coupled receptors (GPCRs) family that interacts with biotic and abiotic stimulants and transmits signals to intracellular downstream pathways in eukaryotic cells. Understanding their role in winter mushroom development is important for cultivation and breeding.

Objective

To investigate the structure and expression patterns of pheromone receptor-like genes (PRLGs) in Winter Mushroom Flammulina filiformis and understand their role in fruiting body development.

Results

The structure of PRLGs was found to be conserved in F. filiformis strains except for some single-nucleotide polymorphism sites. The genes contained 2-5 introns, with varying positions across strains. Three conserved motifs were identified in most PRLGs, though FfSte3.s6 contained only two types. Expression analysis showed that FfSte3.s1 and FfSte3.s2 had maximum expression in mycelia stage, while other PRLGs showed high expression in fruiting body stages.

Conclusion

PRLGs appear to be vital genes involved in fruiting body development in F. filiformis. The variation in expression patterns at different developmental stages suggests distinct roles in mushroom development, though specific functional pathways require further investigation.
  • Published in:Archives of Microbiology,
  • Study Type:Laboratory Research,
  • Source: 10.1007/s00203-020-01990-0
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