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Biosourcing and optimization of fungal lipase production from cheap agro waste via solid state fermentation

Summary

Researchers discovered a fungus called Aspergillus oryzae that produces lipase, an important enzyme used in many industries. They found that this fungus works best when grown on cheap agricultural waste materials like wheat bran and rice bran, making enzyme production more affordable and environmentally friendly. By optimizing growth conditions and using waste materials, they successfully increased lipase production and showed this approach could be used in industrial-scale enzyme manufacturing.

Background

Lipases are important industrial enzymes ranking third in global demand. Microbial lipases, particularly from fungi, offer advantages over plant and animal sources including easier genetic manipulation and cost-effective production. This study focuses on identifying efficient fungal lipase producers using inexpensive agro-wastes as substrates for sustainable enzyme production.

Objective

To screen, identify, and produce lipase from fungi using inexpensive agro-wastes as substrates through solid-state fermentation. The study aimed to determine the effect of medium composition and various agro-waste carbon sources on lipase production by the selected fungal isolate.

Results

Isolate GP11, identified as Aspergillus oryzae (MN416218), exhibited the highest lipase activity. Medium composition significantly affected lipase production, with glucose and peptone supporting highest production (274.05 ± 13.70 U/mL). Among agro-wastes tested, wheat bran and rice bran supported the highest lipase production at 230.78 ± 7.79 and 211.62 ± 3.20 U/mL respectively. The purified lipase achieved 12.74-fold purification with a molecular weight of 53 kDa.

Conclusion

Aspergillus oryzae GP11 is an efficient lipase producer suitable for industrial applications using cheap agro-wastes. Medium composition significantly influences lipase production, with glucose-peptone media and wheat/rice bran as optimal substrates. This approach provides economically viable and sustainable lipase production with reduced environmental impact through waste valorization.
  • Published in:Scientific Reports,
  • Study Type:Experimental Research,
  • Source: 10.1038/s41598-025-06505-9, PMID: 40596505
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