Bioluminescence Expression During the Transition from Mycelium to Mushroom in Three North American Armillaria and Desarmillaria Species

Summary

This research explores how certain mushroom species can produce their own light (bioluminescence) and how this ability changes as they develop from thread-like growth (mycelium) to mature mushrooms. The study found that these fungi gradually lose their ability to glow as they mature, but this can be temporarily reversed by adding specific compounds. Impacts on everyday life: • Helps understand natural light production in organisms, which could inspire development of bio-based lighting solutions • Provides insights into fungal development and metabolism that could be useful for mushroom cultivation • Advances our knowledge of natural biological processes that could lead to biotechnology applications • Contributes to understanding forest ecology where these fungi play important roles • Could lead to new methods for detecting and monitoring wood-decay fungi in forestry

Background

Bioluminescence, the production of visible light by living organisms, has been documented in at least 800 genera worldwide. Among fungi, 77 species exhibit bioluminescence, restricted to four lineages of white-spored mushroom producing Basidiomycota. Uniquely in the Armillaria lineage, only mycelial bioluminescence has been documented, with no light production observed in mature mushrooms.

Objective

The study aimed to understand the inactivation of bioluminescence in Armillaria and Desarmillaria mushrooms by examining how light production changes during mycelium to mushroom transition, and whether adding luciferin precursor hispidin could enhance or induce light production in different growth stages.

Results

A tenfold decline in luminescence was observed during the transition from mycelia to immature mushrooms in Armillaria species. As pins matured, luminescence further declined by two to three orders of magnitude. D. tabescens showed lower initial luminescence that declined to negligible levels during development. Light production was localized in the gills and lower stipe of A. mellea mushrooms. Adding hispidin enhanced luminescence by one to three orders of magnitude in stipe and gills respectively.

Conclusion

The modulation of Armillaria and Desarmillaria luminescence is achieved through luciferin availability early in mushroom development. The unique temporal regulation of bioluminescence in Armillaria species compared to other genera suggests distinct selective pressures on this trait.
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