MYCOLab Ai Logo - gold and army green

Bioinformatics Analysis, Expression Profiling, and Functional Characterization of Heat Shock Proteins in Wolfiporia cocos

Summary

This research investigated how medicinal fungi respond to high temperatures by studying special proteins called heat shock proteins. These proteins help organisms survive in hot conditions. The study provides important insights into how fungi adapt to temperature stress, which could help improve cultivation practices. Impacts on everyday life: • Better understanding of how to grow medicinal mushrooms in different climates • Improved methods for commercial mushroom cultivation • Potential applications in developing heat-resistant crops • Insights into how organisms naturally protect themselves from heat stress • Applications for preserving beneficial fungi in changing climate conditions

Background

Heat shock proteins (HSPs) play critical roles in regulating different mechanisms under high-temperature conditions. While HSPs have been well-studied in plants, there is limited information about their genomic organization and roles in medicinal fungi, particularly Wolfiporia cocos.

Objective

To identify and characterize heat shock proteins in W. cocos, analyze their expression patterns under different temperature conditions, and determine their functional role in heat stress tolerance.

Results

Sixteen HSPs were identified and classified into five subgroups (WcHSP100, WcHSP90, WcHSP70, WcHSP60, and WcsHSP). Expression profiling revealed most HSPs were induced by high temperature, with different subfamilies showing distinct expression patterns. WcHSP70-4, WcHSP90-1, and WcHSP100-1 showed the highest response to high temperature stress. Heterologous expression of these genes in E. coli enhanced bacterial survival rate during heat stress.

Conclusion

The study provides comprehensive insights into the functional characterization of W. cocos heat shock proteins and establishes their role in heat stress tolerance. WcHSP100-1, WcHSP90-1, and WcHSP70-4 were identified as key genes in responding to high temperature stress in W. cocos.
  • Published in:Bioengineering (Basel),
  • Study Type:Laboratory Research,
  • Source: 10.3390/bioengineering10030390
Scroll to Top