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Biodecolorization and Biodegradation of Methyl Orange by Immobilized Pseudomonas aeruginosa Bacterium into SA/PVA Matrix Integrated with MOF UiO-66 Adsorbent

Summary

Scientists created special beads containing bacteria and a porous material called MOF to remove orange dye (methyl orange) from contaminated water. The bacteria break down the dye molecules while the porous material helps trap the dye, working together more effectively than either method alone. These beads removed over 92% of the dye from water in just 10 days, making them promising for treating textile industry wastewater.

Background

Azo dyes like methyl orange (MO) are persistent pollutants with environmental and health concerns. Pseudomonas aeruginosa has demonstrated capability to degrade MO, but free-cell forms lack stability. Immobilization in polymeric matrices with metal-organic frameworks could enhance treatment efficiency.

Objective

This study aimed to develop an integrated system combining immobilized Pseudomonas aeruginosa with UiO-66 metal-organic framework embedded in sodium alginate/polyvinyl alcohol matrix for enhanced biodecolorization and biodegradation of methyl orange dye.

Results

SA/PVA/UiO-66/PA beads achieved 92.03% MO removal, 33.99% higher than free bacterial cells. UiO-66 incorporation enhanced adsorption capacity by 20.47% and 43.5% improvement in maximum adsorption capacity compared to SA/PVA alone. LC-MS identified nine biodegradation products, confirming structural breakdown of MO.

Conclusion

The integrated SA/PVA/UiO-66/PA system successfully combined bacterial biodegradation with MOF adsorption, significantly enhancing MO removal efficiency. The pseudo-second-order kinetics and Langmuir isotherm model dominated the adsorption process, demonstrating chemisorption mechanisms and practical applicability for dye-contaminated wastewater treatment.
  • Published in:Biotechnology Reports,
  • Study Type:Experimental Research,
  • Source: 10.1016/j.btre.2025.e00924, PMID: 41368516
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