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Biocontrol Potential of Bacillus velezensis RS65 Against Phytophthora infestans: A Sustainable Strategy for Managing Tomato Late Blight

Summary

Researchers tested 30 soil bacteria from tomato roots to find natural disease fighters. They discovered that Bacillus velezensis RS65, a beneficial bacterium, effectively prevents tomato late blight by producing enzymes and toxins that kill the fungal pathogen. In greenhouse tests, plants treated with RS65 had half the disease severity compared to untreated plants, suggesting this bacteria could replace chemical fungicides in sustainable tomato farming.

Background

Tomato late blight caused by Phytophthora infestans is a major destructive disease affecting global tomato production. Chemical fungicides used for control pose environmental and health risks while promoting pathogen resistance. Rhizospheric bacteria, particularly Bacillus species, have shown promise as sustainable biocontrol alternatives for disease management.

Objective

To investigate the biocontrol activity of rhizospheric bacterial isolates against P. infestans in tomatoes and identify the most effective antagonistic strain. The study aimed to evaluate both in vitro and in vivo antagonistic mechanisms and provide a sustainable alternative to chemical fungicides.

Results

Six isolates showed significant inhibition (>67%) of P. infestans mycelial growth in vitro, with RS65 exhibiting the highest inhibition rate of 78.48%. RS65 demonstrated significant enzymatic activities and HCN production. Under greenhouse conditions, RS65 treatment reduced disease severity from 31.26% (control) to 16.54%. Molecular identification confirmed RS65 as Bacillus velezensis with 99% genetic proximity.

Conclusion

Bacillus velezensis RS65 shows significant biocontrol potential against tomato late blight and could serve as an effective biopesticide in integrated disease management programs. The strain’s combination of enzymatic activities and HCN production provides multiple antagonistic mechanisms. Field trials and formulation development are recommended for practical agricultural application.
  • Published in:Microorganisms,
  • Study Type:Experimental Study,
  • Source: PMID: 40142548, DOI: 10.3390/microorganisms13030656
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