Beneficial bacterial-Auricularia cornea interactions fostering growth enhancement identified from microbiota present in spent mushroom substrate

Summary

Researchers discovered that certain beneficial bacteria, particularly Pseudonocardia mangrovi, can significantly boost the growth of wood ear mushrooms (Auricularia cornea) through laboratory studies. By analyzing the microscopic communities in spent mushroom substrates from high-yielding versus low-yielding farms, they identified bacteria that promote mushroom growth through multiple mechanisms. Co-cultivation experiments and protein analysis revealed these bacteria work synergistically by helping mushrooms break down nutrients and produce growth-enhancing compounds. This research can help farmers select beneficial microbes to improve mushroom yields and profitability.

Background

Auricularia cornea (wood ear mushroom) is the third most cultivated mushroom globally. Complex bacterial-fungal interactions play key roles during mushroom cultivation, ranging from mutualistic to antagonistic relationships that influence mycelial and fruiting body formation.

Objective

To identify growth-promoting bacteria from spent mushroom substrates of high-yield versus low-yield Auricularia cornea cultivations using high-throughput amplicon sequencing, and to elucidate the molecular mechanisms underlying beneficial bacterial-fungal interactions.

Results

Five bacterial species showed high differential abundance between high and low-yield groups: Pseudonocardia mangrovi, Luteimonas composti, Paracoccus pantotrophus, Sphingobium jiangsuense, and Microvirga massiliensis. Co-cultivation of A. cornea with P. mangrovi promoted the highest mycelial growth. Proteomics analysis identified 1,616 total proteins (578 from A. cornea, 1,038 from P. mangrovi) involved in carbohydrate metabolism, peptidase activity, and secondary metabolite biosynthesis.

Conclusion

Pseudonocardia mangrovi acts as a growth-promoting bacterium for A. cornea through concerted actions of carbohydrate-active enzymes, peptidases, transporters, and metabolites including indoles, terpenes, β-lactones, and iturins. These findings enable selection of beneficial bacteria to improve A. cornea cultivation yield and productivity.
Scroll to Top