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Attenuation of Inflammatory Mediators (TNF-α and Nitric Oxide) and Up-Regulation of IL-10 by Wild and Domesticated Basidiocarps of Amauroderma rugosum in LPS-Stimulated RAW264.7 Cells

Summary

This research examined how a traditional medicinal mushroom called Amauroderma rugosum can help reduce inflammation and act as an antioxidant. The study found that both wild and cultivated forms of the mushroom effectively reduced inflammation markers and increased anti-inflammatory compounds in cells. This validates its traditional use in Asian medicine. Impacts on everyday life: • Provides scientific evidence supporting traditional medicine practices • Demonstrates potential for developing natural anti-inflammatory treatments • Shows that cultivated mushrooms can be as effective as wild ones • Offers a potential natural alternative to synthetic anti-inflammatory drugs • Suggests new ways to help manage inflammatory conditions naturally

Background

Amauroderma rugosum is a wild mushroom traditionally used in Chinese medicine to reduce inflammation, treat diuretic and upset stomach conditions, and prevent cancer. In Malaysia, indigenous communities use it to prevent epileptic episodes and incessant crying in babies. Inflammation and oxidative stress are linked to various diseases, making the study of natural anti-inflammatory compounds important.

Objective

To compare the antioxidant and in vitro anti-inflammatory effects of wild and domesticated basidiocarps of A. rugosum in LPS-stimulated RAW264.7 cells, and to validate its traditional medicinal uses.

Results

Both wild (WB) and domesticated (DB) basidiocarps showed comparable total phenolic content and DPPH scavenging activity, though WB displayed better ABTS radical scavenging activity. Both extracts suppressed NO production through down-regulation of iNOS gene expression, reduced TNF-α levels, and increased anti-inflammatory IL-10 production. Neither extract showed inhibitory effects on NF-κB p65 nuclear translocation.

Conclusion

The study validated the traditional anti-inflammatory uses of A. rugosum, with both wild and domesticated forms showing significant antioxidant and anti-inflammatory properties. The mushroom’s ability to attenuate proinflammatory mediators while increasing anti-inflammatory cytokine production suggests potential therapeutic applications in inflammatory conditions.
  • Published in:PLoS One,
  • Study Type:In Vitro Study,
  • Source: 10.1371/journal.pone.0139593
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