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Antioxidant and Enzyme Inhibitory Potential of Streptomyces sp. G-18 Grown in Various Media

Summary

Researchers studied bacteria called Streptomyces that were isolated from high mountains in Nepal to see if they could produce useful compounds. They grew these bacteria in four different types of growth media and tested the resulting extracts for antioxidant properties and ability to block harmful enzymes. They found that the choice of growth medium significantly affected what compounds the bacteria produced, with one medium (R2YE) being especially effective at producing compounds that could help treat diseases like Alzheimer’s and diabetes.

Background

Streptomyces bacteria are well-known producers of bioactive secondary metabolites with diverse biochemical properties including antifungal, antiviral, and antibiotic activities. The biosynthesis of these metabolites is influenced by growth medium, environmental conditions, and gene regulation. This study investigated how different growth media affect the production of bioactive compounds in Streptomyces sp. G-18 isolated from high altitude soil in Nepal.

Objective

To investigate the influence of different growth media on biomass production and the antioxidant and enzyme inhibitory potential of crude extracts from Streptomyces sp. G-18. The study evaluated four different media (R2YE, YEME, R5, and R5M) for their effects on metabolite production.

Results

R2YE medium showed the highest dry weight (184 mg/L) and highest alpha-amylase inhibition (66%, IC50=130±0.5 μg/mL). R2YE extracts exhibited the strongest antioxidant activity with 82% ABTS scavenging. YEME medium showed the best lipase inhibition (45%, IC50=157±6.0 μg/mL), while only R2YE demonstrated elastase and acetylcholinesterase inhibition.

Conclusion

Streptomyces sp. G-18 represents a promising source of enzyme inhibitors with potential pharmaceutical and cosmetic applications. The production of bioactive secondary metabolites was significantly influenced by growth medium selection, with R2YE and R5 media being most suitable for optimal biological activities.
  • Published in:International Journal of Microbiology,
  • Study Type:Experimental Study,
  • Source: PMID: 37583475, DOI: 10.1155/2023/6439466
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