MYCOLab Ai Logo - gold and army green

Antibiofilm activity and bioactive phenolic compounds of ethanol extract from the Hericium erinaceus basidiome

Summary

Researchers tested a lion’s mane mushroom extract for its ability to stop harmful bacteria from forming protective biofilms that make infections harder to treat. The extract showed strong activity against Proteus mirabilis bacteria, reducing biofilm formation by over 78%. The antibiofilm power comes mainly from two phenolic compounds: protocatechuic acid and p-coumaric acid. These findings suggest lion’s mane mushroom could be developed into natural supplements or food preservatives to prevent bacterial contamination.

Background

Biofilm formation by pathogenic bacteria poses serious health and environmental challenges due to their high antibiotic resistance. Mushrooms are recognized as valuable sources of bioactive compounds with antimicrobial properties. The lion’s mane mushroom (Hericium erinaceus) has been traditionally used as an antimicrobial agent in East Asian countries, though studies on its antibiofilm activities remain limited.

Objective

To determine the antibiofilm activity of Hericium erinaceus ethanol extract against biofilm-forming pathogenic bacteria and to identify its phenolic compound profile using HPLC-MS analysis.

Results

H. erinaceus exhibited antibiofilm activities against all tested bacteria, with P. mirabilis showing the highest susceptibility (78.18% biofilm inhibition). The total phenolic content was 1652±1.06 µg/ml, with protocatechuic acid (352.94 µg/ml) and p-coumaric acid (42.05 µg/ml) as the major phenolic compounds. Despite weak direct antibacterial activity, the extract demonstrated significant antibiofilm potential.

Conclusion

The ethanol extract of H. erinaceus basidiome has potential as an antibiofilm agent that could be developed as a nutraceutical or natural food preservative, with protocatechuic acid and p-coumaric acid contributing to its bioactive properties.
  • Published in:Journal of Advanced Pharmaceutical Technology & Research,
  • Study Type:Experimental study,
  • Source: PMID: 35464662
Scroll to Top