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Ammonia Fiber Expansion Combined with White Rot Fungi to Treat Lignocellulose for Cultivation of Mushrooms

Summary

This research demonstrates an improved method for converting agricultural waste into valuable mushroom products using a combination of chemical treatment and fungal cultivation. The process makes waste materials more digestible for mushroom-producing fungi while reducing processing time and increasing yields. This has several practical implications: • More efficient recycling of agricultural waste into food products • Increased mushroom production yields for farmers • Reduced environmental impact from agricultural waste disposal • More sustainable food production methods • Potential cost savings in mushroom cultivation

Background

China produces over 800 million tons of agricultural waste annually, accounting for one-third of global waste. Most agricultural and forestry wastes contain valuable lignocellulose that could replace fossil resources, but its complex structure makes decomposition and utilization difficult. Current physical, chemical and biological treatment methods each have limitations in effectively processing lignocellulose.

Objective

To develop an improved method for treating lignocellulose by combining ammonia fiber expansion (AFEX) pretreatment with white rot fungi cultivation, aiming to reduce treatment time while increasing lignocellulose degradation efficiency and mushroom yields.

Results

The combined AFEX and fungal treatment improved lignocellulose degradation rates by 10-20% compared to single treatments. P. geesteranus showed the best degradation ability, with degradation rates of 35.56% for cellulose, 33.40% for hemicellulose, and 36.65% for lignin when combined with AFEX treatment. The treatment reduced mycelial growth time by 25% and increased mushroom yields by 44.6%. Enzyme activities were significantly enhanced, with peak activities of 2195.67 U/g for laccase, 258.538 U/g for manganese peroxidase, and 91.18 U/g for lignin peroxidase.

Conclusion

The combined use of AFEX pretreatment with white rot fungi cultivation provides an effective method for lignocellulose degradation, reducing treatment time while improving degradation efficiency and mushroom yields. This approach shows promise for better utilization of agricultural waste materials.
  • Published in:ACS Omega,
  • Study Type:Laboratory Research,
  • Source: 10.1021/acsomega.1c04388
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