Abies sachalinensis Acquires Iron Tolerance Through Multiple Mechanisms Including Root Endophytic Phialocephala bamuru
- Author: mycolabadmin
- 2025-06-17
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Summary
This research reveals how Sakhalin fir trees can naturally survive in soil heavily contaminated with iron from mining operations. The trees use multiple strategies to cope with toxic iron levels, including producing protective compounds in their roots and maintaining helpful relationships with beneficial fungi. This discovery has important real-world implications:
• Provides a natural solution for cleaning up and restoring abandoned mine sites
• Offers an environmentally friendly alternative to using invasive plant species
• Demonstrates how native plants and their microbial partners can be used for environmental restoration
• Shows potential for developing more effective plant-based methods to clean up metal-contaminated soils
• Could help improve forest restoration practices in areas affected by mining activities
Background
After mine operations cease, vegetation restoration is important to prevent heavy metals spread from sedimentary sites. While invasive species are traditionally used, native plant species are preferred for ecological diversity. Sakhalin fir (Abies sachalinensis) naturally grows at heavy metal-rich sedimentary sites in Hokkaido, Japan, but its heavy metal tolerance mechanisms were previously unstudied.
Objective
To clarify the heavy metal tolerance mechanisms of A. sachalinensis and examine the effects of root endophytes on this tolerance, with a focus on understanding how this conifer species survives in heavy metal-contaminated environments.
Results
A. sachalinensis accumulated high levels of iron (>2,000 mg/kg dry weight) in fine roots throughout the sampling period. The roots contained malic acid, catechin, and condensed tannins which can detoxify iron. The Fe/Mn ratio was significantly lower in leaves compared to roots, suggesting regulated metal transfer. Among isolated root endophytes, Phialocephala bamuru showed the highest siderophore production activity, which could help detoxify iron in the host plant.
Conclusion
A. sachalinensis demonstrates multiple iron tolerance mechanisms including production of iron detoxicants (malic acid, catechin, condensed tannins) and regulation of Fe/Mn ratios between roots and leaves. Additionally, the root endophyte P. bamuru appears to enhance iron tolerance through siderophore production. These combined mechanisms allow A. sachalinensis to survive in heavy metal-contaminated environments, making it potentially useful for ecological restoration of mining sites.
- Published in:PLoS One,
- Study Type:Field Study,
- Source: 10.1371/journal.pone.0325294