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A Water-Soluble Polysaccharide from Grifola frondosa Induced Macrophages Activation via TLR4-MyD88-IKKβ-NF-κB p65 Pathways

Summary

This research investigated how a compound extracted from the Grifola frondosa mushroom can boost immune system function. The study found that this mushroom-derived substance can activate immune cells called macrophages, making them more effective at defending the body against potential threats. Impacts on everyday life: • Could lead to development of natural immune-boosting supplements • May help create new treatments for immune system disorders • Demonstrates potential health benefits of consuming medicinal mushrooms • Could help develop safer alternatives to current immunotherapy drugs • Provides scientific backing for traditional uses of mushrooms in medicine

Background

Polysaccharides are widely present in living organisms and have various biological functions including immune regulation, anti-viral, anti-oxidant, anti-tumor, hypoglycemic and lipid-lowering effects. Grifola frondosa, a mushroom marketed in Asian countries, contains bioactive compounds including polysaccharides that have attracted attention for their various therapeutic properties.

Objective

To investigate the immunomodulatory effects of water-soluble polysaccharide from Grifola frondosa (GFPS) on RAW264.7 macrophages and elucidate its molecular mechanisms of action.

Results

GFPS enhanced immunostimulatory activity by increasing NO release and cytokine production. It elevated TLR4 expression which acted as an upstream regulator of MyD88. MyD88 promoted IKKβ in endochylema and translocated NF-κB p65 subunit into the nucleus, increasing NO production and cytokine/chemokine levels. GFPS also increased lysozyme, SOD and POD enzyme activities in treated cells.

Conclusion

GFPS demonstrates potent immunostimulatory activity in RAW264.7 macrophages by activating the TLR4-MyD88-IKKβ-NF-κB p65 signaling pathway. It stimulates enzyme activities and induces production of NO and inflammatory mediators, confirming its potential as an immunomodulatory agent.
  • Published in:Oncotarget,
  • Study Type:Laboratory Research,
  • Source: 10.18632/oncotarget.21252
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