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A Two-Step Bioconversion Process for Canolol Production from Rapeseed Meal Combining an Aspergillus Niger Feruloyl Esterase and the Fungus Neolentinus Lepideus

Summary

This research developed an environmentally-friendly way to produce canolol, a valuable antioxidant compound, from rapeseed meal waste using fungi and enzymes. The process is more efficient than traditional methods and avoids harsh chemicals. Impact on everyday life: – Enables sustainable production of natural antioxidants for food and health products – Provides a way to create value from agricultural waste materials – Demonstrates greener manufacturing processes that avoid toxic chemicals – Could lead to more affordable antioxidant supplements and food preservatives – Supports development of natural alternatives to synthetic additives

Background

Rapeseed meal is a cheap and abundant raw material, particularly rich in phenolic compounds like sinapic acid. Canolol, derived from sinapic acid, has valuable antioxidant, antimutagenic and anticarcinogenic properties with potential industrial applications. Current methods to obtain canolol from rapeseed meal involve harsh chemical conditions incompatible with green chemistry.

Objective

To develop a two-step bioconversion process to produce canolol from rapeseed meal using two complementary fungi – Aspergillus niger and Neolentinus lepideus – under mild conditions compatible with green chemistry principles.

Results

The AnFaeA enzyme achieved 68-76% global hydrolysis yield and 100% hydrolysis of sinapine from the meal, releasing 6.6-7.4 mg of free sinapic acid per gram of raw meal. The subsequent bioconversion by N. lepideus produced 1.3 g/L canolol with 80% molar yield and productivity of 100 mg/L per day. The overall process yielded 3.3 mg canolol per gram of rapeseed meal, representing a 4.1-6.6 fold increase compared to traditional heat treatments.

Conclusion

This study successfully developed the first fungal/enzymatic bioconversion process for producing canolol from rapeseed meal under mild conditions suitable for sustainable biotechnology applications. The two-step process using complementary fungi achieved higher yields than conventional methods while maintaining green chemistry principles.
  • Published in:Microorganisms,
  • Study Type:Laboratory Research,
  • Source: 10.3390/microorganisms5040067
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