A Single Transcription Factor (PDD1) Determines Development and Yield of Winter Mushroom (Flammulina velutipes)

Summary

This research identified a key genetic factor that controls mushroom development and growth in the commercially important winter mushroom. By manipulating this gene (PDD1), researchers were able to increase mushroom yields and speed up cultivation time. This discovery has important implications for mushroom farming and production. Impacts on everyday life: • Could lead to more efficient mushroom production and lower costs for consumers • May help increase the availability of nutritious mushrooms in the food supply • Demonstrates potential for improving mushroom farming through genetic approaches • Could enable cultivation of currently uncultivatable mushroom species • May contribute to more sustainable food production methods

Background

Mushrooms are important sources of food and medicine, providing nutrients and bioactive compounds. However, most edible mushrooms cannot be cultivated commercially due to limited understanding of basidioma (fruiting body) development. Winter mushroom (Flammulina velutipes) is one of the most commonly cultivated mushrooms globally.

Objective

To identify and characterize the role of a novel transcription factor, PDD1, in regulating basidioma development and mushroom yield in Flammulina velutipes.

Results

PDD1 overexpression promoted basidioma formation and significantly increased mushroom yield by at least 33%. The cultivation time was shortened by 9.8% in overexpression mutants. PDD1 knockdown dramatically impaired mushroom development. RNA sequencing revealed PDD1 regulates 794 genes, including ones important for basidioma development like pheromone receptors and jacalin-related lectins.

Conclusion

PDD1 is the first identified transcription factor with defined functions in mushroom development among commercially cultivated mushroom species. It positively regulates basidioma development through multiple genetic pathways. The findings suggest PDD1 could be valuable for mushroom breeding to improve yields and cultivation efficiency.
Scroll to Top