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A Putative Transcription Factor LFC1 Negatively Regulates Development and Yield of Winter Mushroom

Summary

This research identified a gene that controls mushroom growth and development in winter mushrooms. By reducing the activity of this gene, researchers were able to make mushrooms grow faster and produce higher yields. This discovery could help mushroom farmers grow more mushrooms more efficiently. Impacts on everyday life: • Could lead to faster growing mushrooms in commercial production • May help reduce the cost of mushrooms for consumers • Could improve mushroom farming efficiency and productivity • May contribute to more sustainable food production methods • Could lead to development of new mushroom varieties with desired characteristics

Background

Basidioma is the fruiting body of mushroom species. Understanding the mechanisms of basidioma development is valuable for mushroom breeding and cultivation. Winter mushroom (Flammulina velutipes) is one of the top five industrially cultivated mushrooms.

Objective

To identify and characterize the role of a novel putative Zn(II)2Cys6 transcription factor LFC1 in regulating basidioma development in winter mushroom.

Results

The transcript level of lfc1 decreased dramatically during basidioma development. Knockdown of lfc1 promoted basidioma development, shortened cultivation time by 2 days, and increased mushroom yield and biological efficiency by at least 24%. Overexpression delayed harvest time by 3 days and caused wrinkled cap edges. RNA-seq analysis revealed 455 genes with opposite transcriptional responses to lfc1 overexpression and knockdown, including hydrophobin genes, lectin genes, and membrane component genes.

Conclusion

LFC1 functions as a negative regulator of basidioma development in winter mushroom. Its manipulation can affect cultivation time, yield, and mushroom morphology. As LFC1 homologs are widely present in mushroom species, lfc1 can be useful in mushroom breeding.
  • Published in:Applied Microbiology and Biotechnology,
  • Study Type:Experimental Research,
  • Source: 10.1007/s00253-020-10642-8
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