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A Novel Gene, Le-DD10, is Involved in Fruiting Body Formation of Lentinula edodes

Summary

This research discovered a new gene that helps control how shiitake mushrooms develop their edible mushroom bodies. The findings could lead to improved mushroom cultivation methods with shorter growing times. Impacts on everyday life: • Could lead to faster-growing shiitake mushroom varieties • May help reduce cultivation costs and increase mushroom production efficiency • Could improve availability and potentially lower costs of shiitake mushrooms for consumers • Advances our understanding of how edible mushrooms grow and develop • May contribute to improved cultivation methods for other mushroom species

Background

Shiitake mushroom (Lentinula edodes) is one of the most common edible basidiomycetes among Japanese cultivated mushrooms. While several genes related to fruiting body development have been isolated in model fungi like Coprinopsis cinerea, identifying genes involved in L. edodes fruiting body formation has been challenging due to its long culture period requirements.

Objective

To identify and characterize novel genes involved in fruiting body formation in Lentinula edodes using Dictyostelium discoideum cDNA as probes, since D. discoideum can form fruiting bodies in 24 hours compared to the 2-3 months required for L. edodes.

Results

The Le-Dd10 cDNA overexpression clones showed shorter periods to reach fruiting body formation compared to other overexpression clones and similar timing to the positive control H600. Le-Dd10 was mapped to linkage group LG4. Western blot analysis revealed that Le-Dd10 produces proteins of 56 kDa, 27 kDa, and 14 kDa in primordial and fruiting body stages, with different protein patterns observed in the mycelial stage. The 14 kDa protein showed strong expression in varieties with shorter culture periods for fruiting body formation.

Conclusion

The study identified Le-Dd10 as a novel gene involved in stimulating fruiting body formation in L. edodes. The Le-Dd10 protein appears to function as a transcription factor, with its activity potentially regulated through the Spt2 domain and modifications of sugar and phosphorylation. The conversion of the 56 kDa protein to a 14 kDa form may be important for stimulating fruiting body formation, suggesting potential applications for developing shorter cultivation period varieties.
  • Published in:Archives of Microbiology,
  • Study Type:Laboratory Research,
  • Source: 10.1007/s00203-022-03206-z
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