A Novel Apoptosis Correlated Molecule: Expression and Characterization of Protein Latcripin-1 from Lentinula edodes C91-3
- Author: mycolabadmin
- 2012-05-21
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Summary
Scientists have discovered a new protein from an edible mushroom that can kill lung cancer cells while leaving normal cells unharmed. This is significant because most current cancer treatments can’t tell the difference between healthy and cancerous cells, leading to side effects. The protein, named Latcripin-1, was isolated from a special strain of shiitake mushroom and works by triggering self-destruction specifically in cancer cells.
Impacts on everyday life:
– Provides hope for developing more targeted cancer treatments with fewer side effects
– Demonstrates the potential medical value of common edible mushrooms
– Shows how natural compounds can be used to fight serious diseases
– Opens new possibilities for lung cancer treatment
– Highlights the importance of preserving and studying natural species for medical research
Background
Cancer is one of the most serious diseases threatening human health worldwide. There is an urgent need to find new drugs with fewer side effects that can improve patient survival rates. Lentinula edodes C91-3 is an edible mushroom isolated from Basidiomycetes Umbelliferae fungi that has shown direct anti-tumor effects in previous research.
Objective
To express and characterize a novel apoptosis-related protein called Latcripin-1 from Lentinula edodes C91-3 and evaluate its potential anti-tumor effects on human lung cancer cells.
Results
The Latcripin-1 protein was successfully expressed and characterized at 118 kDa. MTT assays showed significant inhibition of A549 cancer cell growth in a time and concentration-dependent manner. Flow cytometry confirmed the protein induced apoptosis in A549 cells. Electron microscopy revealed characteristic apoptotic changes in treated cells. Importantly, the protein showed no toxic effects on normal chick embryo fibroblasts.
Conclusion
The novel protein Latcripin-1 from Lentinula edodes C91-3 was successfully expressed and shown to induce apoptosis specifically in human lung cancer cells without harming normal cells. This work provides new insights for finding anti-tumor proteins and establishes a foundation for further studies of Lentinula edodes C91-3’s anti-cancer properties.
- Published in:International Journal of Molecular Sciences,
- Study Type:Laboratory Research,
- Source: 10.3390/ijms13056246