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A New Exopolysaccharide from a Wood-Decaying Fungus Spongipellis borealis for a Wide Range of Biotechnological Applications

Summary

Researchers isolated and studied a new polysaccharide from the wood-decaying mushroom Spongipellis borealis. This polysaccharide is composed mainly of glucose, galactose, and mannose sugars and acts as a natural stabilizer for important enzymes used in biotechnology. When this polysaccharide is added to enzyme preparations, it significantly improves their stability and performance under various conditions, making it promising for industrial and medical applications.

Background

Fungi are rich natural sources of polysaccharides with various biological activities. Spongipellis borealis is a wood-decaying fungus that produces bioactive compounds including polysaccharides. Fungal polysaccharides have been shown to possess immunomodulating, antitumor, and antioxidative properties.

Objective

The main aim of this investigation was to isolate, identify, and structurally characterize a new exopolysaccharide (EPS) derived from laboratory-cultured vegetative mycelium of a new Spongipellis borealis strain. Additionally, the study examined the stabilizing effect of this EPS on proteolytic enzymes and laccase activity.

Results

The EPS was composed mainly of glucose, galactose, and mannose monomers with terminal, →3)-linked, →4)-linked, and →2,4)-linked hexoses. EPS significantly enhanced the stability of proteolytic enzymes (pepsin, trypsin, pycnoporopepsin) and laccase. The presence of EPS improved laccase stability at different temperatures and pH values, and protected laccase activity in the presence of non-ionic surfactants.

Conclusion

The new EPS from S. borealis demonstrates significant potential for biotechnological applications as a natural enzyme stabilizer. The polysaccharide effectively modifies enzyme properties and increases stability of biocatalytically important enzymes. Easy standardization of production conditions and high efficiency make this preparation promising for scaled-up biotechnological processes.
  • Published in:Molecules,
  • Study Type:Original Research,
  • Source: PMID: 37630373, DOI: 10.3390/molecules28166120
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