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A Laccase with HIV-1 Reverse Transcriptase Inhibitory Activity from the Broth of Mycelial Culture of the Mushroom Lentinus tigrinus

Summary

This research identified an enzyme from the mushroom Lentinus tigrinus that shows potential for HIV treatment by blocking a key viral enzyme. The study represents an important step in finding new natural compounds that could help fight HIV infection. Impact on everyday life: – Could lead to new HIV treatment options from natural sources – Demonstrates the medical potential of mushroom compounds – Advances our understanding of how natural enzymes can be used therapeutically – Shows promise for developing new pharmaceutical products from fungi

Background

Laccases are glycosylated multicopper oxidases first discovered in the Japanese tree Rhus vernicifera. They are widely distributed among plants, insects, bacteria, and fungi. In recent years, laccases have been studied for industrial applications including bioremediation and phenolic removal. Lentinus tigrinus is a white rot fungus known for efficient lignin degradation through production of laccases and ligninolytic peroxidases.

Objective

To isolate and characterize a laccase with HIV-1 reverse transcriptase inhibitory activity from the mycelium of L. tigrinus HPXG59 and compare its characteristics with laccases isolated from other L. tigrinus strains.

Results

A 59 kDa laccase was successfully isolated with approximately 95-fold purification and 25.9% yield. The enzyme showed HIV-1 reverse transcriptase inhibitory activity with an IC50 of 2.4μM. Its N-terminal sequence was GIPDLHDLTV, showing little similarity to other mushroom laccases. The enzyme exhibited maximal activity at pH 4 and 60°C. The specific enzyme activity was 76.6 U/mg.

Conclusion

The study successfully isolated and characterized a novel laccase from L. tigrinus with distinct properties from previously reported strains, demonstrating potentially exploitable HIV-1 reverse transcriptase inhibitory activity.
  • Published in:Journal of Biomedicine and Biotechnology,
  • Study Type:Laboratory Research,
  • Source: 10.1155/2012/536725
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