A Homeodomain-Containing Transcriptional Factor PoHtf1 Regulated the Development and Cellulase Expression in Penicillium oxalicum
- Author: mycolabadmin
- 2021-06-10
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Summary
This research investigated how a specific gene regulator (PoHtf1) controls both fungal growth and the production of important industrial enzymes in the fungus Penicillium oxalicum. The findings show that this regulator acts as a master switch controlling both fungal development and enzyme production. Understanding this regulation helps scientists engineer better fungal strains for industrial enzyme production.
Impacts on everyday life:
– Improved production of enzymes used in laundry detergents and textile processing
– More efficient conversion of plant waste into biofuels
– Better understanding of fungal growth control for agricultural applications
– Potential development of more environmentally friendly industrial processes
Background
Homeodomain-containing transcription factors (Htfs) play important roles in animals, fungi, and plants during developmental processes. In fungi, Htfs regulate development and differentiation but their specific roles in cellulase-producing fungi are not well understood.
Objective
To functionally characterize the homeodomain-containing transcription factor PoHtf1 in the cellulase-producing fungus Penicillium oxalicum 114-2 and understand its role in regulating development and cellulase expression.
Results
PoHtf1 deletion significantly impaired colony growth and conidiation through regulation of the key conidiation regulator BrlA. The deletion also enhanced cellulase production by coordinated regulation of cellulolytic regulators CreA, AmyR, ClrB and XlnR. Transcriptome analysis revealed PoHtf1’s involvement in secondary metabolism including conidial yellow pigment synthesis.
Conclusion
PoHtf1 mediates a complex transcriptional-regulatory network between developmental processes and cellulolytic gene expression in P. oxalicum 114-2. It functions as both a developmental regulator and cellulase expression inhibitor. These findings provide insights for metabolic engineering of fungal strains for enhanced enzyme production.
- Published in:Frontiers in Microbiology,
- Study Type:Experimental Research,
- Source: 10.3389/fmicb.2021.671089