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Potential Bioactivities, Chemical Composition, and Conformation Studies of Exopolysaccharide-Derived Aspergillus sp. Strain GAD7

Summary

Scientists discovered a marine fungus called Aspergillus sp. strain GAD7 that produces a special type of sugar-like substance with medicinal properties. This substance can help prevent blood clotting and fight harmful substances in the body that cause damage. The research shows this fungal product could potentially be used as a therapeutic treatment for conditions related to blood clotting and oxidative stress.

Background

Marine fungi are a promising source of bioactive compounds with unique adaptations to environmental conditions. Extracellular polysaccharides (EPS) from fungi have attracted significant research interest for their potential biomedical applications including antioxidant, anti-inflammatory, and anticoagulant properties.

Objective

This study aimed to screen marine fungal isolates for EPS production, characterize the most potent isolate structurally and conformationally, and evaluate its anticoagulant and antioxidant bioactivities.

Results

EPS-AG7 exhibited the highest production yield of 5.19 g/L and was identified as a heterogeneous acidic sulfated polysaccharide with 87.5% carbohydrate and 24% sulfate content. SRCD and Congo Red assays confirmed a triple helix-like conformation stable under various conditions. EPS-AG7 demonstrated significant anticoagulant activity, doubling blood coagulation time at 3.0 mg/mL, and antioxidant activity with 85.90% DPPH scavenging and 58.64% ABTS+ scavenging at 5.0 mg/mL.

Conclusion

EPS-AG7 from Aspergillus sp. strain GAD7 represents a promising candidate for therapeutic applications due to its potent anticoagulant and antioxidant activities combined with a stable triple helix conformation. The comprehensive structural characterization demonstrates the importance of detailed molecular analysis in understanding structure-activity relationships for polysaccharide-based therapeutics.
  • Published in:Journal of Fungi,
  • Study Type:Experimental Research,
  • Source: 10.3390/jof10090659
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