MYCOLab Ai Logo - gold and army green

A simple protocol for producing axenic seeds of Sorghum bicolor

Summary

This study presents a simple and inexpensive method for sterilizing sorghum seeds to remove all living microbes while keeping them viable for planting. The process involves soaking seeds in ethanol and bleach for short periods, followed by rinsing. Of 95 seeds tested, only 2 showed contamination, and over half successfully germinated, making this method practical for seed research.

Background

Microbes within seeds can confound research on microbial colonization, symbiosis, and pathogenesis. Sterilization of both external and internal seed tissues is essential for certain experiments, but the method must preserve seed viability. Existing seed sterilization methods for many plant species often fail to eliminate internal culturable microbes.

Objective

To present a reliable and simple protocol for sterilizing Sorghum bicolor seeds that eliminates both internal and external culturable microbes while maintaining seed viability. The protocol aims to provide a practical, cost-effective solution for experiments requiring axenic seeds.

Results

Of 95 seeds tested, only 2 showed microbial contamination (both from the same student group), suggesting a possible handling error. The median germination rate was 63%, which is reasonable considering the chemical treatment. All other seeds showed no microbial growth after germination.

Conclusion

This protocol reliably eliminates culturable microbes from Sorghum bicolor seeds while preserving viability. It is fast, low-cost, and does not require specialized equipment, making it a practical option for experiments requiring axenic seeds. The method may be broadly adaptable to other plant species.
  • Published in:MicroPubl Biol,
  • Study Type:Protocol Development Study,
  • Source: 10.17912/micropub.biology.001772, PMID: 40994815
Scroll to Top