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Isolation, identification and antibacterial activity of endophytes from the seeds of Panax japonicus

Summary

Researchers isolated and identified 10 different microorganisms (7 fungi and 3 bacteria) living inside Panax japonicus seeds. They found that a specific type of bacteria (Enterobacteriaceae and Pseudomonas) may help promote seed growth, while certain fungi may inhibit it. The study also determined the best way to sterilize seeds before experimentation, which is important for studying plant propagation of this valuable medicinal herb.

Background

Panax japonicus is a traditional medicinal plant with significant pharmacological properties. Seed germination of P. japonicus is difficult due to high contamination rates and after-ripening phenomena. Endophytes from medicinal plants are known to promote plant growth and improve plant quality.

Objective

To isolate and identify endophytic fungi and bacteria from P. japonicus seeds and evaluate their antibacterial activity. To determine optimal seed disinfection conditions and identify endophytes that promote seed germination.

Results

Seven endophytic fungal strains and three endophytic bacterial strains were isolated and identified. Optimal disinfection achieved 3.33% contamination using 75% alcohol for 30s and 1% HgCl2 for 9 min. Only Pj Z4 showed weak inhibitory activity against E. coli, while Pj Z3, Z4, Z5, and Z7 showed activity against B. subtilis. Metabolites of all strains showed no antibacterial activity.

Conclusion

Fusarium spp. and Alternaria alternata are likely inhibitory to seed germination, while Enterobacteriaceae and Pseudomonas sp. are identified as growth-promoting endophytes. Results provide foundational knowledge for future applications in P. japonicus cultivation and seed germination studies.
  • Published in:PLoS One,
  • Study Type:Experimental Study,
  • Source: PMID: 41066337, DOI: 10.1371/journal.pone.0330436
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