Transcriptomic insights into the molecular mechanism of abietic acid promoting growth and branching in Armillaria gallica

Summary

Scientists discovered that abietic acid, a natural compound from pine trees, significantly boosts the growth of Armillaria gallica mushrooms by up to 302% in just three days. This fungus is important because it helps grow Gastrodia elata, a valuable traditional Chinese medicinal herb. By studying which genes turned on in response to abietic acid, researchers found it works by helping the fungus break down nutrients more efficiently and remodel its cell walls for better growth. This discovery could improve cultivation techniques for medicinal mushrooms and their plant partners.

Background

Armillaria gallica is a valuable edible and medicinal fungus essential for symbiotic cultivation of Gastrodia elata, a traditional Chinese medicinal herb. Abietic acid, a plant-derived secondary metabolite from conifers, has known antimicrobial properties but its role in promoting fungal growth remains unexplored. This study investigates how abietic acid influences A. gallica development at the molecular level.

Objective

To elucidate the molecular mechanisms by which abietic acid promotes growth and branching in Armillaria gallica using phenotypic analysis and transcriptomic approaches. The study aims to provide theoretical foundations for enhancing symbiotic cultivation of G. elata and A. gallica.

Results

Abietic acid significantly promoted A. gallica growth and branching, with day 3 showing 302% increase in dry biomass and 378.4% increase in total rhizomorphs (p<0.01). Transcriptomic analysis identified 4,945 differentially expressed genes on day 3, with upregulation of GH5, GH16, MFS, and NAD(P)-binding protein genes. Enzyme assays confirmed 193.6% increase in GH5 activity and 107.2% increase in GH16 activity.

Conclusion

Abietic acid promotes A. gallica growth through optimized carbon utilization, cell wall remodeling, and nutrient transport mechanisms. The findings provide molecular evidence for abietic acid’s growth-promoting effects and offer strategies for enhancing symbiotic cultivation of medicinal fungi and their host plants.
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