Insights into the special physiology of Mortierella alpina cultured by agar supported solid state fermentation in enhancing arachidonic acid enriched lipid production

Summary

Researchers developed a new fermentation method using agar plates to grow a fungus called Mortierella alpina that produces arachidonic acid, an important nutrient used in medicines and supplements. This method produced significantly more arachidonic acid than traditional liquid fermentation while being more environmentally friendly. By understanding how the fungus grows differently on agar plates, they optimized the production process to achieve even higher yields, making this approach promising for commercial production.

Background

Arachidonic acid (ARA) is a polyunsaturated fatty acid with important biological functions used in pharmaceuticals, nutrition, and cosmetics. Submerged fermentation (SmF) is the conventional method for ARA production from Mortierella alpina, but solid-state fermentation (SSF) offers environmental advantages.

Objective

To demonstrate that agar-supported SSF (AgSF) can enhance arachidonic acid-rich lipid production in Mortierella alpina M0223 compared to submerged fermentation and to elucidate the underlying physiological mechanisms through comparative transcriptome analysis.

Results

AgSF-M0223 produced 74.26% higher lipid content and 72.53% ARA composition compared to SmF, with total yields of 7.28 g/L lipids containing 4.38 g/L ARA. Transcriptome analysis revealed that surface hyphae exhibited attenuated citrate cycle coupled with enhanced triglyceride biosynthesis and NADPH supply. Optimization through nutrient spraying strategy increased lipid yield to 12.64 g/L with 70.41% ARA content.

Conclusion

Agar-supported solid-state fermentation represents a promising eco-friendly paradigm for ARA-rich lipid production from Mortierella alpina, achieving significantly higher yields than conventional submerged fermentation while providing new insights into fungal physiology under SSF conditions.
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