Haplotype-Phased Chromosome-Level Genome Assembly of Floccularia luteovirens Provides Insights into Its Taxonomy, Adaptive Evolution, and Biosynthetic Potential

Summary

Scientists successfully decoded the complete genetic blueprint of the yellow mushroom (Floccularia luteovirens), a valuable medicinal fungus found on the Tibetan Plateau. The high-quality genome assembly revealed the mushroom produces many different beneficial compounds like antitumor and anti-inflammatory molecules. The study also corrected previous scientific confusion about the mushroom’s evolutionary classification, showing it’s more closely related to other fungi than previously thought, and revealed how it adapted to harsh alpine conditions.

Background

Floccularia luteovirens is a valuable medicinal and edible ectomycorrhizal fungus endemic to alpine meadows of the Qinghai-Tibet Plateau. Previous genome assemblies were fragmented with poor continuity, limiting understanding of its genomic features, taxonomy, and biosynthetic potential.

Objective

To generate a high-quality, haplotype-phased chromosome-level genome assembly of F. luteovirens QHU-1 strain and elucidate its taxonomy, adaptive evolution mechanisms, and secondary metabolite biosynthetic capacity.

Results

Successfully assembled a 26.77 Mb haplotype-phased genome across 13 chromosomes with 97.6% BUSCO completeness. Phylogenomic analysis clarified F. luteovirens diverged from Armillaria ~90.6 MYA and is most closely related to Mycocalia denudata/Crucibulum laeve. Identified 15 biosynthetic gene clusters encoding 7 terpenoid synthases, 4 NRPS-like enzymes, 2 PKSs, and 1 hybrid PKS-NRPS synthase with potential for novel bioactive compounds.

Conclusion

This chromosome-level genome resource resolves taxonomic ambiguity, reveals genomic adaptations to extreme alpine conditions through stable transposable elements and strong purifying selection, and unlocks the biosynthetic potential of F. luteovirens for bioprospecting bioactive compounds and understanding its ectomycorrhizal symbiosis.
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