Multi-omics analysis of Taiwanofungus gaoligongensis: effects of different cultivation methods on secondary metabolites
- Author: mycolabadmin
- 8/1/2025
- View Source
Summary
Researchers studied how different growing methods affect the medicinal compounds produced by Taiwanofungus gaoligongensis, a rare fungus. By growing the fungus on different substrates including wood from specific trees, they found that certain growing methods produced much higher levels of beneficial compounds like antcins that have anti-cancer and anti-inflammatory properties. They also identified which genes control the production of these compounds, which could help improve cultivation methods to make the fungus more medicinally valuable.
Background
Taiwanofungus gaoligongensis is a newly identified fungal species with bioactive secondary metabolites including triterpenoids and antcins. Different cultivation methods significantly affect the production of these bioactive compounds, which have important medicinal properties.
Objective
This study investigated the effects of various cultivation methods on secondary metabolite production in T. gaoligongensis using multi-omics analysis, including fruiting bodies on wood logs and mycelia on different substrates.
Results
NZJB and XZJB significantly enhanced terpenoid and triterpenoid production, with antcin C reaching 9.72-fold higher levels in XZJB and antcin I 12.83-fold higher in NZJB compared to DM. TgHMGR expression was markedly elevated in NZJB and correlated with higher terpenoid levels, suggesting it acts as a key rate-limiting enzyme.
Conclusion
Cultivation on C. kanehirae and C. camphora substrates in fungal cultivation bags significantly promotes bioactive compound accumulation compared to rice medium. Transcriptional regulation by transcription factors TgHSF4, TgMYB6, and others was identified as controlling key biosynthetic genes, providing strategies to enhance medicinal compound production.
- Published in:Frontiers in Microbiology,
- Study Type:Experimental Research,
- Source: PMC12355660, 40822405