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Genome sequence of the novel Cystobasidiomycetes fungal isolate EMM_F5

Summary

Researchers isolated and sequenced the genome of a previously unclassified yeast called EMM_F5 found on Magnolia tree leaves. Through genetic analysis, they determined this yeast belongs to the Cystobasidiomycetes class and likely represents a new family called Microsporomycetaceae. This discovery fills an important gap in fungal genomics by providing the first genetic information available for this family of fungi.

Background

Yeasts are known colonizers of the phyllosphere habitat, though many remain unclassified. EMM_F5 was isolated from the phyllosphere of Magnolia grandiflora at Auburn University in 2021. The isolate displayed yellow-orange colonies with butyrous texture and yeast-like morphology.

Objective

To sequence, assemble, and annotate the whole genome of fungal isolate EMM_F5 and perform phylogenomic analysis to determine its taxonomic placement within Basidiomycota fungi.

Results

The final assembled genome was 18,587,251 bp with 253 contigs and 52% GC content, achieving 86.4% BUSCO completeness. Phylogenomic analysis placed EMM_F5 within Cystobasidiomycetes class, with multigene phylogenies clustering it with Microsporomyces follicola, suggesting placement in Microsporomycetaceae family.

Conclusion

EMM_F5 represents the first genomic resource for Microsporomycetaceae family, highlighting a significant gap in fungal genomics. This work provides valuable genomic data for an unclassified yeast species from the phyllosphere habitat.
  • Published in:Microbiology Resource Announcements,
  • Study Type:Genome Sequencing and Annotation,
  • Source: 10.1128/mra.00718-25, PMID: 41159974
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