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Antiviral effects of Pediococcus acidilactici isolated from Tibetan mushroom and comparative genomic analysis

Summary

Researchers found that bacteria from Tibetan mushrooms, particularly Pediococcus acidilactici, can effectively prevent rotavirus infection that causes diarrhea in children and animals. When tested in laboratory cells and in mice, this beneficial bacteria reduced virus levels significantly and protected the intestines from damage. Genetic analysis shows this probiotic strain has the right combination of genes to fight viral infections, making it a promising natural treatment option.

Background

Rotavirus is a major pathogen causing diarrhea in young animals and infants worldwide. Tibetan mushrooms are rich in lactic acid bacteria with probiotic properties. This study investigated the antiviral potential of lactic acid bacteria isolated from Tibetan mushrooms against rotavirus infection.

Objective

To isolate and characterize lactic acid bacteria from Tibetan mushrooms, evaluate their probiotic properties and antiviral effects against rotavirus, and perform comparative genomic analysis of the most effective strain.

Results

Pediococcus acidilactici, Lactobacillus casei, and Lactobacillus paracasei were identified. Pediococcus acidilactici demonstrated superior acid tolerance, bile salt tolerance, and pathogenic bacteria inhibition. In vitro and in vivo experiments showed Pediococcus acidilactici significantly reduced rotavirus replication and protected intestinal epithelial integrity. Whole genome analysis revealed 2,026,809 bp with 1,988 genes and 50.6% glycoside hydrolases.

Conclusion

Pediococcus acidilactici isolated from Tibetan mushrooms shows excellent antiviral properties against rotavirus through both in vitro and in vivo models. Genomic analysis confirms its potential as a candidate probiotic strain for preventing rotavirus-induced diarrhea. Further development of this strain as a therapeutic product for viral diarrhea prevention is warranted.
  • Published in:Frontiers in Microbiology,
  • Study Type:Experimental Study,
  • Source: PMID: 36704546
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