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Isolation, Characterization, and Wound-Healing Potential of β-D-Glucan from Lycoperdon pyriforme Schaeff

Summary

Researchers isolated and studied a compound called β-D-glucan from puffball mushrooms (Lycoperdon pyriforme) that have been traditionally used to heal wounds and stop bleeding. Laboratory tests showed this compound safely promotes the movement and growth of skin cells that help wounds close without harming red blood cells, and it actually helps blood clot faster. These findings support the traditional use of this mushroom and suggest it could be developed into a new natural wound-healing treatment.

Background

Lycoperdon pyriforme is a puffball mushroom traditionally used by local communities for wound healing and hemostasis. β-Glucans from fungi have demonstrated immunoregulatory, anti-inflammatory, and wound-healing properties in recent scientific investigations.

Objective

To isolate, purify, and characterize β-D-glucan from Lycoperdon pyriforme and evaluate its wound-healing potential, hemocompatibility, and cytotoxic effects.

Results

GPC analysis revealed two peaks with molecular weights of 3 kDa and 104.55 kDa indicating polydispersity. Spectroscopic analyses confirmed β-(1→3) and β-(1→6) glycosidic linkages. The isolated β-D-glucan showed no cytotoxicity at concentrations up to 1000 μg/mL and promoted fibroblast migration with 40-50% wound closure at 10 hours at moderate concentrations. Hemolytic activity remained below 5% (ASTM E2524-08 safe threshold) and blood coagulation index was below 10%, indicating pro-coagulant properties.

Conclusion

β-D-glucan from Lycoperdon pyriforme demonstrates non-toxic, hemocompatible, and wound-healing properties supporting its traditional medicinal use. The compound’s ability to promote cell migration, enhance blood coagulation, and maintain hemolytic safety suggests potential therapeutic applications in wound care and hemostasis.
  • Published in:ChemistryOpen,
  • Study Type:In Vitro Experimental Study,
  • Source: PMID: 40717328, DOI: 10.1002/open.202500131
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