Microbiome Analysis Reveals Biocontrol of Aspergillus and Mycotoxin Mitigation in Maize by the Growth-Promoting Fungal Endophyte Colletotrichum tofieldiae Ct0861
- Author: mycolabadmin
- 10/22/2025
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Summary
Researchers discovered that a beneficial fungal endophyte called Colletotrichum tofieldiae can protect maize crops from contamination by harmful Aspergillus fungi that produce dangerous toxins called aflatoxins. When maize plants were treated with this endophyte either through seed coating or leaf spraying, they grew better and produced higher yields while also experiencing a dramatic 90% reduction in fungal contamination and extremely low aflatoxin levels. The protection mechanism appears to work indirectly by boosting the plant’s own defense systems rather than through direct combat with the pathogenic fungus, offering a sustainable and safe solution for improving crop quality and food safety.
Background
Maize is a globally critical crop threatened by mycotoxigenic fungi like Aspergillus spp., which contaminate grains with harmful mycotoxins. Fungal endophytes offer sustainable strategies to improve plant resilience against biotic stresses. Colletotrichum tofieldiae strain Ct0861 has previously demonstrated growth-promotion capabilities in multiple plant species.
Objective
This study evaluates the potential of Colletotrichum tofieldiae strain Ct0861 as a bioinoculant and assesses its impact on maize-associated bacterial and fungal microbiomes. The research aims to determine whether Ct0861 can reduce Aspergillus spp. abundance and mycotoxin levels while preserving native microbiome stability.
Results
Ct0861 enhanced maize biomass and yield by 21-22% regardless of application method. Microbiome analysis revealed significant reduction in Aspergillus spp. abundance in treated plants, with qPCR confirming 90% reduction in A. flavus biomass at 5 days post-infection and 83% at 21 days. Aflatoxin B1 levels decreased from 72 µg/kg in controls to 0.46 µg/kg in treated plants.
Conclusion
Ct0861 acts as an effective biocontrol agent against mycotoxigenic Aspergillus spp. in maize through an indirect mechanism likely involving plant-induced resistance, as direct antagonism was not observed. The endophyte enhances food safety by reducing mycotoxin accumulation without disrupting the native microbiome, supporting its use as a sustainable crop protection tool.
- Published in:Plants (Basel),
- Study Type:Field Trial with Greenhouse Validation,
- Source: PMID: 41225787