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Novel and advanced MNP molecular markers accurately identify the genetic similarity of Hypsizygus marmoreus strains: a comparative evaluation with ISSR and antagonistic methods

Summary

Scientists developed a new DNA-based method to accurately identify different strains of king oyster mushrooms (Hypsizygus marmoreus). Using advanced genetic analysis on 32 mushroom varieties, they created a database of 369 genetic markers that can distinguish between strains with high accuracy. This new method is faster and more reliable than traditional testing methods, helping mushroom farmers and breeders maintain quality and prevent confusion between similar-looking varieties.

Background

Hypsizygus marmoreus is an industrially cultivated wood-rotting fungus with significant medicinal and nutritional value. As production scales up, accurate identification of strains has become increasingly important to address the issue of identical strains bearing different names. Traditional morphological and molecular methods have limitations in reproducibility and efficiency.

Objective

To develop and validate multiple nucleotide polymorphism (MNP) molecular markers for accurate identification and genetic similarity assessment of H. marmoreus strains. The study aims to establish an MNP marker database and compare its effectiveness with ISSR and antagonistic methods for strain differentiation.

Results

No completely genetically identical strains were found among the 32 selected varieties. White strains showed genetic similarity ranging from 11.92% to 88.62%, while gray strains ranged from 2.71% to 74.53%, indicating greater genetic diversity in gray strains. MNP markers demonstrated superior stability, accuracy, and efficiency compared to antagonism tests and ISSR methods.

Conclusion

The MNP molecular marker method successfully identified and differentiated H. marmoreus strains with high accuracy and reproducibility. The established 369 MNP marker database provides a reliable tool for variety identification and strain authentication in the edible fungi industry, significantly advancing breeding efforts and commercial development.
  • Published in:Frontiers in Fungal Biology,
  • Study Type:Comparative Analytical Study,
  • Source: PMID: 41209487
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