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Statistical methodologies for enhancing lipase production from Aspergillus Niger and using biologically treated cottonseed waste in animal nutrition

Summary

This study shows how scientists can grow an enzyme called lipase on cottonseed waste using a fungus called Aspergillus niger. The leftover treated material becomes excellent animal feed with high protein and important amino acids. This approach solves two problems at once: producing valuable enzymes for industry while creating nutritious feed for livestock from agricultural waste.

Background

Lipases are industrially valuable biocatalysts with applications in pharmaceuticals and food industries. Cottonseed waste, a byproduct of cottonseed oil production, represents an abundant agro-industrial resource that could serve dual purposes in enzyme production and animal nutrition. The study addresses the gap between available and required animal feed in developing countries.

Objective

To optimize lipase production from Aspergillus niger MK377324 using cottonseed waste as substrate through statistical design methods and evaluate the nutritional value of biologically treated residual biomass for animal feed applications.

Results

Cottonseed waste proved optimal for lipase production, yielding 21.42 ± 1.3 U/g dry substrate with peptone, K2HPO4, and CuSO4 supplementation. The treated residual biomass showed increased crude protein content from 26.1% to 29.6%, with significant essential amino acids including lysine (1.28%), threonine (1.04%), and methionine (0.44%), while crude fiber decreased from 18.3% to 15.6%.

Conclusion

Using cottonseed waste as both substrate for lipase production and as animal feed represents a promising dual-purpose solution addressing environmental and nutritional challenges. The biological treatment significantly improves the nutritional value of cottonseed waste for ruminant feed while enabling efficient enzyme production for industrial applications including biodiesel production.
  • Published in:Scientific Reports,
  • Study Type:Experimental Study,
  • Source: 10.1038/s41598-025-19731-y, PMID: 41057463
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