Taxonomy and Phylogeny of Cystolepiota (Agaricaceae, Agaricales): New Species, New Combinations and Notes on the C. seminuda Complex

Summary

Researchers studied tiny mushrooms in the genus Cystolepiota using DNA analysis and microscopy to better understand their relationships and identify new species. They discovered that what was thought to be a single species called C. seminuda was actually multiple different species. They also found that another group of mushrooms called Pulverolepiota was distinct enough to deserve its own genus rather than being classified with Cystolepiota. This work clarifies the true diversity and proper classification of these diminutive fungi found worldwide.

Background

Cystolepiota comprises diminutive lepiotaceous fungi with worldwide distribution. Previous molecular studies revealed the genus is not monophyletic, and recent DNA sequence data suggested the existence of several new species. The C. seminuda complex in particular has shown considerable taxonomic controversy due to variability in basidiomata size, color, and spore morphology.

Objective

To re-delimit Cystolepiota and discuss its relationships with related genera, typify and circumscribe C. seminuda based on recent specimens, and explore species diversity within Cystolepiota by describing novel species based on morphological characteristics and multi-locus DNA sequence data.

Results

Two new species C. pseudoseminuda and C. pyramidosquamulosa were described. The C. seminuda complex was revealed to contain at least three species. Genus Pulverolepiota was resurrected with two new combinations: P. oliveirae and P. petasiformis. C. seminuda was re-circumscribed and neo-typified based on recent collections from near its type locality.

Conclusion

Pulverolepiota forms a distinct generic-level clade separate from Cystolepiota, warranting resurrection as an independent genus. The C. seminuda complex represents a species complex with at least three distinct species that can be differentiated by morphological and molecular characteristics. The study clarifies systematic positions and describes novel fungal diversity within the Agaricaceae.
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