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Chromosome-Wide Characterization of Intragenic Crossover in Shiitake Mushroom, Lentinula edodes

Summary

This study examines how genetic material is shuffled during reproduction in shiitake mushrooms. Scientists identified 65 regions in the mushroom genome where genetic crossovers happen more frequently. They discovered that special DNA sequences and specific genes regulate where these crossovers occur. Understanding these patterns can help improve mushroom breeding programs by making it easier to combine desirable traits.

Background

Meiotic crossover plays a critical role in generating genetic variations and is essential for breeding programs. While crossover mechanisms have been extensively studied in model organisms, understanding in mushroom-forming fungi remains limited. This study addresses the gap by characterizing chromosome-wide intragenic crossovers in Lentinula edodes using SNP datasets from F1 haploid progeny.

Objective

To characterize the genome-wide distribution of intragenic crossovers in Lentinula edodes, identify crossover hotspots, determine their genetic architecture, and understand the cis- and trans-regulation of crossover variation.

Results

A total of 884 intragenic crossovers were identified, with 71.5% clustered into 65 crossover hotspots. A 10 bp motif (GCTCTCGAAA) was enriched in hotspot regions. Sixteen crossover-QTLs were identified, contributing 8.5-29.1% of variations, with both cis- and trans-acting factors contributing to crossover nonuniformity. A QTL hotspot on chromosome 2 regulated local and global crossover variation.

Conclusion

This study provides comprehensive characterization of the crossover landscape in L. edodes and advances understanding of conservation and diversity of meiotic recombination in mushroom-forming fungi. The findings demonstrate complex genetic regulation of crossover through both cis- and trans-loci and identify specific chromosomal regions controlling recombination rates.
  • Published in:Journal of Fungi,
  • Study Type:Genetic and Molecular Study,
  • Source: PMID: 34947058, DOI: 10.3390/jof7121076
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