Utilization of Corn Steep Liquor for the Production of Fairy Chemicals by Lepista sordida Mycelia

Summary

Researchers found that corn steep liquor, a waste product from corn processing, can be used as an inexpensive ingredient to help grow mushroom mycelia that produce fairy chemicals. Fairy chemicals are special compounds with potential uses in agriculture and cosmetics, but they are very expensive to produce. By using different concentrations of corn steep liquor in growth media, scientists determined optimal conditions for either growing more mycelia or producing more of the beneficial chemicals, making these compounds more affordable for practical use.

Background

Fairy chemicals (FCs) consisting of 2-azahypoxanthine (AHX), imidazole-4-carboxamide (ICA), and 2-aza-8-oxohypoxanthine (AOH) have potential applications in agriculture, cosmetics, and medicine. However, high production costs limit their practical use. Food industrial by-products such as corn steep liquor (CSL), rice bran, wheat bran, and Japanese liquor lees could serve as cost-effective nutritional additives for fungal cultivation.

Objective

To determine optimal culture conditions for reducing production costs of fairy chemicals and mycelia of Lepista sordida by evaluating food industrial by-products as nutritional additives in liquid culture media.

Results

CSL was more effective than other tested additives for increasing both mycelial yield and fairy chemical production. Medium with 1% CSL was optimal for mycelial yield (1.77 times control), while 6% CSL was optimal for fairy chemical production (8.58 times AHX and 3.79 times ICA compared to control). Nutrient stress from different CSL concentrations appeared to influence the balance between mycelial growth and metabolite production.

Conclusion

CSL-1% medium is optimal for obtaining high mycelial yield while CSL-6% medium is optimal for high fairy chemical production. This research provides practical methods for cost-effective production of fairy chemicals and mycelia using agricultural by-products, facilitating their commercial application in agriculture, cosmetics, and medicine.
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