The Impact of Insecticides on Mycelial Growth of Metarhizium spp. and Their Efficacy in Controlling Larvae and Pupae of the House Fly (Musca domestica L.)
- Author: mycolabadmin
- 7/31/2025
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Summary
This study tested fungal organisms called Metarhizium as a natural way to control house flies without relying solely on chemical insecticides. Researchers found that two fungal isolates were very effective at killing house fly larvae and pupae. Importantly, these fungi could still work well even when grown alongside certain pyrethroid insecticides, making them useful for integrated pest management approaches that combine multiple control methods.
Background
House flies (Musca domestica L.) are significant pests that transmit over 100 types of pathogens affecting humans and animals. Chemical insecticides are commonly used for control but insects develop resistance. Metarhizium spp. are entomopathogenic fungi with potential as biological control agents in integrated pest management systems.
Objective
This study evaluated 10 Metarhizium spp. isolates for effectiveness against house fly larvae and pupae, and tested the compatibility of the most effective isolates with insecticides to assess impact on mycelial growth and pest control efficacy.
Results
NM Met_SS9/2 and NM Met_CLPK4/1 showed infection rates of 86.67% and 60.00% for larvae and pupae respectively. Both isolates grew well on PDA with Cypas® 250 EC at 50 mL per 6.25 L (81.33 mm and 77.67 mm colony diameter) without significant differences from PDA alone. Spore suspensions from these media achieved 93.33% and 75.56% mortality rates with comparable efficacy to controls.
Conclusion
NM Met_SS9/2 and NM Met_CLPK4/1 are highly effective against house fly larvae and pupae and can proliferate on media containing Cypas® 250 EC at half the recommended rate without compromising insecticidal properties, making them promising candidates for integrated pest management strategies.
- Published in:Tropical Life Sciences Research (Trop Life Sci Res),
- Study Type:Experimental Laboratory Study,
- Source: 10.21315/tlsr2025.36.2.6, PMID: 41244239