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Simultaneous Detection of Four Madurella Species Using Loop-Mediated Isothermal Amplification (LAMP) for Eumycetoma Diagnosis

Summary

Scientists developed a simple new test to identify four types of fungi that cause a serious tropical disease called eumycetoma. Unlike previous tests that required expensive equipment and multiple reactions, this new test works in one tube using simple heating instead of complex thermal cycling. The test can detect the fungi in about 45 minutes and works reliably in remote areas with limited resources.

Background

Eumycetoma is a neglected tropical disease primarily caused by Madurella mycetomatis and related Madurella species. Early diagnosis is challenging due to initial subtle symptoms, leading to misdiagnosis and high morbidity. Existing PCR-based detection methods require expensive equipment unsuitable for low-resource rural settings.

Objective

To develop a novel LAMP primer set capable of simultaneously detecting all four known Madurella species in a single reaction. This aims to create a simpler, more cost-effective diagnostic tool for eumycetoma that is suitable for rural clinical settings.

Results

All three primer sets detected up to 1 pg of genomic DNA from all four Madurella species with no cross-reactivity to other pathogenic fungi. Primer #1 (targeting MMYC01_204841) showed optimal performance with consistent reactivity across all Madurella species at 66°C reaction temperature with approximately 45-minute detection time.

Conclusion

A single LAMP primer set was successfully developed to simultaneously detect all known Madurella species in one reaction, eliminating the need for multiple separate reactions. This advancement simplifies diagnostic protocols, reduces costs, and should enable broader implementation in resource-limited settings.
  • Published in:Mycopathologia,
  • Study Type:Experimental/Molecular Diagnostic Development Study,
  • Source: 10.1007/s11046-025-01019-4; PMID: 41152542
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