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Study on Optimization of Liquid Fermentation Medium and Antitumor Activity of the Mycelium on Phyllopora lonicerae

Summary

Scientists optimized how to grow Phylloporia lonicerae fungus in the lab, increasing its production by 142% while cutting growth time by two-thirds. They discovered that certain compounds extracted from this fungus, particularly from a petroleum ether extract, can kill cancer cells and trigger apoptosis. The petroleum ether extract was more effective than the standard cancer drug 5-fluorouracil, especially against esophageal cancer cells, suggesting this fungus could be developed into a natural anti-cancer treatment.

Background

Phylloporia lonicerae is an annual fungus that parasitizes Lonicera plants and has potential as a food and medicinal resource. Wild resources are limited and mycelium production is low, with unclear anti-tumor mechanisms hampering development of this fungal resource.

Objective

This study aimed to optimize the liquid fermentation medium for P. lonicerae and investigate the anti-tumor activity of its mycelium extract against various cancer cell lines.

Results

The optimized medium achieved 7.82 g/l biomass in 15 days (142% increase over standard medium, 64% reduction in cultivation time). The intracellular alcohol extract showed higher inhibitory effects on A549 and Eca-109 cells with IC50 values of 2.42 and 2.92 mg/ml respectively. Petroleum ether phase exhibited superior anti-tumor activity with IC50 of 113.3 μg/ml and induced apoptosis in Eca-109 cells.

Conclusion

The optimized fermentation medium significantly improved P. lonicerae biomass production with reduced cultivation time. Petroleum ether components demonstrated promising anti-cancer potential, particularly against esophageal cancer cells, suggesting clinical applications in functional foods and cancer treatment development.
  • Published in:Journal of Microbiology and Biotechnology,
  • Study Type:Experimental Research,
  • Source: 10.4014/jmb.2405.05004, PMID: 39187449
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