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The complete mitochondrial genome of the banana pathogen Fusarium oxysporum f. sp. cubense M5

Summary

Researchers have completed the full genetic map of the mitochondria (energy-producing structures) of a fungus that causes a serious disease in banana plants. The study identified all the genes in this mitochondrial genome and discovered special structures called introns within two genes. This genetic information could help scientists develop better ways to detect and identify this harmful banana pathogen.

Background

Fusarium oxysporum f. sp. cubense is the causal agent of fusarium wilt, a disease that hampers banana production worldwide. Fungal mitochondrial DNA presents high variation in gene number and position, though F. oxysporum shows low intra-species variation with three variants of a large variable region.

Objective

To report the complete mitochondrial genome sequence of F. oxysporum f. sp. cubense M5 isolated from diseased banana plants in southern Mexico and identify all genes encoding proteins related to respiration, ATP synthesis, rRNAs, and tRNAs.

Results

The complete circular mitochondrial genome assembly is 45,675 bp with 32.1% GC content. It harbors 18 protein-coding genes (2 of unknown function), 2 rRNA-encoding genes, and 26 tRNA-encoding genes, all encoded on the sense strand. Two Group I introns were identified in nad5 and rnl genes, with heg1 and rps3 encoded within them respectively.

Conclusion

The complete mitochondrial genome of Foc M5 has been successfully sequenced and annotated, providing a resource for understanding this banana pathogen. The identified variable regions may enable development of novel PCR-based diagnostic strategies for fusarium wilt.
  • Published in:Microbiology Resource Announcements,
  • Study Type:Genome Sequencing and Annotation Study,
  • Source: 10.1128/mra.00421-24; PMID: 39248489
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