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A Chromosome-Scale Genome of Trametes versicolor and Transcriptome-Based Screening for Light-Induced Genes That Promote Triterpene Biosynthesis

Summary

Scientists created a detailed map of the Trametes versicolor mushroom’s genetic code using advanced sequencing technologies. This medicinal mushroom is known for cancer-fighting and immune-boosting properties. The research discovered that light exposure increases the production of beneficial compounds called triterpenes, which may explain how this mushroom’s medicinal qualities work and could help scientists grow it more effectively.

Background

Trametes versicolor is an important medicinal fungus with significant immunomodulatory, antitumor, and anti-inflammatory properties. Despite its vast potential in medicine and biotechnology, the molecular genetic mechanisms and genomic features remain insufficiently explored. Understanding its genome is crucial for uncovering complex metabolic product synthesis and environmental adaptability.

Objective

To construct a high-quality chromosome-level genome of T. versicolor and identify light-induced genes that promote triterpene biosynthesis through transcriptomic analysis. The study aims to provide comprehensive genomic resources for investigating functional genomics, metabolic regulation, and triterpene biosynthesis.

Results

A 47.42 Mb high-quality genome with 13,307 protein-coding genes was assembled with 95.80% completeness. Phylogenetic analysis identified T. pubescens as the closest relative. Analysis revealed 424 CAZymes, 192 CYP450 genes, 2577 transporter proteins, and 33 secondary metabolite clusters including 2 light-sensitive triterpene biosynthesis clusters. Light exposure significantly increased triterpene content but reduced biomass accumulation.

Conclusion

This study provides a comprehensive genomic resource for T. versicolor with identification of light-sensitive triterpene biosynthesis pathways. The chromosome-scale genome and transcriptomic data will facilitate future research into functional genomics, metabolic regulation, and biotechnological applications of this medicinal fungus.
  • Published in:Journal of Fungi,
  • Study Type:Genome assembly and comparative genomics study,
  • Source: PMID: 39852500
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