Accessory Chromosome Contributes to Virulence of Banana Infecting Fusarium oxysporum Tropical Race 4
- Author: mycolabadmin
- 9/12/2025
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Summary
Scientists studied a dangerous fungal disease that destroys banana crops by examining a special extra chromosome found in the pathogen Fusarium oxysporum Tropical Race 4. They created mutant fungi without this chromosome and found that while the mutants could still grow normally in the lab, they became much less dangerous to banana plants. This discovery shows that this particular chromosome contains genes that help the fungus attack and infect bananas, suggesting potential new ways to combat this devastating crop disease.
Background
Filamentous fungi have evolved compartmentalized genomes with conserved core regions and dynamic accessory regions that drive adaptation to changing environments. Tropical Race 4 (TR4) is a devastating lineage of banana-infecting Fusarium species that causes Fusarium wilt epidemics in the industrial Cavendish banana cultivar. TR4 contains a single accessory chromosome (chromosome 12) which in some strains has undergone extensive internal duplications.
Objective
To investigate the contribution of accessory chromosome 12 (AC12) to virulence of banana-infecting Fusarium oxysporum Tropical Race 4. The study aimed to determine whether AC12 is essential for vegetative growth and plant infection.
Results
AC12 loss mutants were viable and showed normal vegetative growth on standard media, indicating AC12 is dispensable for vegetative growth. Plants infected with AC12 loss mutants showed significantly reduced corm necrosis (48.9% compared to 95% in parental strain). Benomyl treatment induced interchromosomal rearrangements of core chromosomes, though these did not affect vegetative growth.
Conclusion
Accessory chromosome 12 is dispensable for vegetative growth but contributes significantly to virulence of TR4 on banana plants and tolerance to environmental stresses. The findings highlight the importance of accessory chromosomes in niche adaptation and virulence of the banana-infecting Fusarium TR4 lineage.
- Published in:Molecular Plant Pathology,
- Study Type:Experimental Study,
- Source: PMID: 40939022, DOI: 10.1111/mpp.70146