Metagenomics and In Vitro Growth-Promoting Experiments Revealed the Potential Roles of Mycorrhizal Fungus Humicolopsis cephalosporioides and Helper Bacteria in Cheilotheca humilis Growth

Summary

Scientists studied a rare white plant called Cheilotheca humilis that cannot make its own food through photosynthesis and instead relies on fungi to survive. Using advanced DNA sequencing and laboratory experiments, they discovered that a special fungus called Humicolopsis cephalosporioides and several types of helpful bacteria work together to provide the plant with essential carbon and nutrients. This research reveals how these invisible microbial partners make it possible for this unusual plant to grow and thrive.

Background

Myco-heterotrophic plants like Cheilotheca humilis lack photosynthesis and rely entirely on fungal symbiosis for carbon acquisition. The mycorrhizal fungi and microbial communities supporting the growth of this plant species remain poorly understood, despite their ecological importance.

Objective

This study aimed to identify the microbial community and key microbial species associated with Cheilotheca humilis growth using metagenomics sequencing and in vitro experiments to determine their roles in providing carbon sources and nutrients.

Results

Analysis identified 48 phyla with Pseudomonadota and Actinomycetota as dominant. The mycorrhizal fungus Humicolopsis cephalosporioides was isolated and shown to produce cellulase. Fourteen medium and high-quality MAGs were recovered, with Rhizobium tubonense and Pseudomonas batumici containing genes for carbon fixation and nitrogen transformation pathways.

Conclusion

The mycorrhizal fungus H. cephalosporioides and mycorrhizal helper bacteria such as Rhizobium and Pseudomonas genera play critical roles in supporting Cheilotheca humilis growth through cellulase production, carbon fixation, nitrogen fixation, and phosphate solubilization, providing novel insights into myco-heterotrophic plant survival strategies.
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