Talaromyces pinophilus Strain HD25G2 as a Novel Biocontrol Agent of Fusarium culmorum, the Causal Agent of Root and Crown Rot of Soft Wheat

Summary

A fungus called Talaromyces pinophilus shows promise as a natural pest controller against Fusarium culmorum, which damages wheat crops worldwide. When applied early to wheat seeds, this beneficial fungus produces enzymes that break down the harmful fungus’s cell walls, completely preventing damage and toxin contamination. However, timing is critical—if applied too late, it can paradoxically increase toxin production, suggesting it works best as a preventative treatment before infection occurs.

Background

Fusarium culmorum causes root and crown rot in soft wheat, resulting in significant crop losses globally. The fungus produces mycotoxins including zearalenone (ZEA), posing food safety risks. Chemical fungicides are increasingly scrutinized due to health and environmental concerns, prompting investigation of biological control alternatives.

Objective

To investigate the biocontrol potential of Talaromyces pinophilus HD25G2 against Fusarium culmorum through examination of mycelial growth inhibition, mycotoxin production, and cell wall-degrading enzyme activity.

Results

T. pinophilus reduced F. culmorum growth by over 55% in filtrate assays and 72-73% in dual culture. The biocontrol strain produced high levels of chitinase (1.72 ± 0.02 IU), protease (0.49 ± 0.01 IU), and cellulase. Early T. pinophilus inoculation prevented 100% of F. culmorum growth and ZEA production, though simultaneous inoculation enhanced ZEA production.

Conclusion

T. pinophilus shows promise as a biocontrol agent against F. culmorum due to production of cell wall-degrading enzymes. However, early inoculation strategy is critical to prevent ZEA accumulation. Further research is needed to optimize application strategies and minimize mycotoxin contamination in soft wheat.
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