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Hydrolytic Enzymes in the Secretome of the Mushrooms P. eryngii and P. ostreatus: A Comparison Between the Two Species

Summary

Oyster and king trumpet mushrooms produce various useful enzymes when grown on agricultural waste. Researchers compared the types and amounts of enzymes produced by two mushroom species at different growth stages. The king trumpet mushroom (P. eryngii) produced more enzymes overall than oyster mushrooms (P. ostreatus). These enzymes can be recovered from leftover mushroom substrate and used in various industries like food, pharmaceuticals, and waste treatment.

Background

Pleurotus species are excellent producers of extracellular enzymes and can be cultivated on various substrates containing lignocellulosic materials. These white-rot fungi secrete hydrolytic enzymes for degrading cellulose, hemicellulose, lignin and proteins, making spent mushroom substrate a valuable source for enzyme recovery in biotechnological applications.

Objective

To analyze and compare eleven hydrolytic enzymes in the secretomes of P. eryngii and P. ostreatus collected at three different growth stages and to determine the temporal dynamics and functional profiles of these enzymes under axenic cultivation conditions.

Results

P. eryngii exhibited consistently higher enzymatic activities than P. ostreatus across all measured parameters. Both species showed similar temporal profiles with early-stage reliance on starch degradation, shifting to lignocellulose degradation during full colonization. Proteases remained high during fruiting body formation, while cellulase and hemicellulase activities peaked at full colonization and decreased thereafter.

Conclusion

Both mushroom species release a broad spectrum of hydrolytic enzymes with potential biotechnological applications. The findings emphasize the value of spent mushroom substrate as a source of industrially relevant enzymes and support the development of circular bioeconomy approaches through enzyme recovery and valorization of cultivation waste.
  • Published in:Molecules,
  • Study Type:Comparative Experimental Study,
  • Source: PMID: 40572471, DOI: 10.3390/molecules30122505
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