Fungal alkaloids mediate defense against bruchid beetles in field populations of an arborescent ipomoea

Summary

Certain morning glory trees form partnerships with special fungi that produce protective chemicals called alkaloids. These chemicals are made by the fungi and travel through the plant to the seeds, where they protect them from seed-eating beetles. Trees with more effective fungal partners produce higher levels of these protective chemicals and suffer less damage from the beetles, demonstrating a remarkable example of how plants and fungi work together to survive in nature.

Background

Several Convolvulaceae species harbor heritable fungal endophytes that produce alkaloids translocated to plant tissues. The arborescent morning glory Ipomoea murucoides forms symbiotic relationships with fungal endophytes, but evidence for their ecological role in defense remains incomplete.

Objective

To identify fungal endophytes in Ipomoea murucoides, quantify alkaloid concentrations in leaves and seeds, and evaluate the defensive role of fungal alkaloids against bruchid beetle herbivory in wild populations.

Results

Most trees harbored Ceramothyrium fungi (Chaetothyriales) while some harbored Truncatella (Xylariales). Seeds had higher swainsonine concentrations than leaves. Trees with Ceramothyrium exhibited significantly higher seed swainsonine levels and less bruchid damage compared to Truncatella-harboring trees. Leaf herbivory by chewing insects did not differ between fungal symbionts.

Conclusion

Fungal-derived swainsonine provides defensive protection against seed predators, with Ceramothyrium producing higher alkaloid concentrations and conferring greater protection than Truncatella. Ipomoea murucoides provides a valuable system for studying vertical and horizontal fungal symbiont transmission.
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