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Antagonistic potential and analytical profiling of plant probiotic bacteria using chromatography and mass spectrometry techniques against Botrytis cinerea and Fusarium oxysporum

Summary

Researchers discovered that certain beneficial bacteria can fight plant diseases caused by harmful fungi. Two bacteria strains showed exceptional ability to inhibit the growth of disease-causing fungi that damage crops. These bacteria produce natural compounds like phenols and organic acids that help plants grow better and resist diseases. This research suggests these bacteria could be used as natural alternatives to chemical pesticides for sustainable farming.

Background

Plant probiotics are bacteria that enhance plant growth and health through various mechanisms. Understanding their interactions with host plants requires comprehensive assessment of antagonistic activity and chemical composition. This study evaluated plant probiotic bacteria for their biocontrol potential against common fungal plant pathogens.

Objective

To assess the antagonistic properties of plant probiotic bacterial strains against Botrytis cinerea and Fusarium oxysporum and to identify their bioactive chemical compounds using analytical techniques. The study aimed to validate the potential of certain bacteria as plant probiotics through both antagonistic testing and chemical characterization.

Results

Bacillus rugosus strain SPB7 and Lactobacillus pasteurii DSM 23907 showed the strongest antagonistic activity with inhibition zones of 11-25 mm against Botrytis cinerea and 12-19 mm against Fusarium oxysporum. HPLC and GC-MS identified phenolic compounds (phloroglucinol, gentisic acid, gallic acid, caffeic acid) in B. rugosus and organic acids (formic, acetic, propionic, lactic acid) in L. pasteurii. FT-IR confirmed the presence of hydroxyl groups and carboxyl groups.

Conclusion

The study demonstrates that Bacillus rugosus and Lactobacillus pasteurii produce phenolic and organic acid compounds respectively, which contribute to their antagonistic activity and plant growth-promoting properties. These bacteria show promise as biocontrol agents and plant probiotics for sustainable agriculture. Further evaluation in pilot trials and field applications is recommended to develop effective formulations for plant disease protection.
  • Published in:Bioresour Bioprocess,
  • Study Type:Experimental Research,
  • Source: 10.1186/s40643-025-00853-0, PMID: 40542243
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